Translational_Unit

Part:BBa_K4161508:Design

Designed by: Lingrui Lai   Group: iGEM22_DKU   (2022-07-26)


488aa recombinant protein, Aga2 + ipaD


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 935
    Illegal PstI site found at 800
    Illegal PstI site found at 940
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 935
    Illegal NheI site found at 857
    Illegal PstI site found at 800
    Illegal PstI site found at 940
    Illegal NotI site found at 961
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 935
    Illegal BamHI site found at 920
    Illegal XhoI site found at 968
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 935
    Illegal PstI site found at 800
    Illegal PstI site found at 940
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 935
    Illegal PstI site found at 800
    Illegal PstI site found at 940
    Illegal AgeI site found at 70
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1681


Design Notes

We expect this sequence to have the ability to express a fused protein containing ipaD antigen on the surface of S. cerevisiae. Therefore, the ipaD gene can be either fused with a signaling sequence targeting the plasmic membrane or a protein that natrually exists on the membrane. We also expect the expressed protein be able to be purified so that we can do further testing. In order to prevent the protein-protein interaction from disrupting the normal function of Aga2 and ipaD or disturbing the binding between HA-anti HA antibodies, we add linker sequences between these proteins.


Source

The ipaD gene comes from Shigella genomic sequence and the Aga2 gene comes from S. cerevisiae genomic sequence.

References